Fig. 4

Clonogenic Assay. a. ADF cells were exposed to solvent(s) (Control), 8.5 μM SI113, 0.5 μM QC or their association for 48 h and then allowed to grow and form colonies for the subsequent 12 d. Cell colonies, after staining with crystal violet (left), were counted and the values reported as percent colony number in the histogram (right). b. U373MG cells treated as in panel a, except for the use of SI113 at a concentration of 4.5 μM. c. T98G cells treated as in panel b, except for the use of QC at a concentration of 1 μM. d. GBM3-Luc cells were exposed to solvent(s) (Control), 4 μM SI113, 1 μM QC or their association for 48 h and then allowed to grow and form colonies for the subsequent 26 d. Cell colonies were observed and photographed in phase contrast microscopy (left), counted and the values reported as percent colony number in the histogram (right). In these panels, statistical analysis among groups was done using the One-way ANOVA test followed by the Tukey’s Multiple Comparison Test (* significance vs. Control; ^ significance vs. SI113; § significance vs. QC)