Fig. 5

Analysis of the autophagic process. ADF, U373MG, T98G, GBM3-Luc and GBM-I (Panels a to e) GBM cells were incubated with SI113, QC or their combination using the drug doses employed in Fig. 4 for 48 h and then assayed via western blot for LC3 and p62, as determinants of the autophagic process. Semi-quantitative assessment of LC3 II and p62 amounts, normalized against the β-actin content, is reported for each cell line. kDa = apparent molecular mass