Fig. 6

FOXA1 promotes colon cancer cell migration by increasing enhancer activity of CYR61. a CYR61 protein levels were increased 48 h after transfection with GV219-mCYR61 in HCT116 cells. b CYR61 ectopic expression increased HCT116 cell migration. c CYR61 protein levels were decreased after transfection with CYR61 siRNA for 48 h in RKO cells. d Knockdown of CYR61 decreased RKO cell migration. e CYR61 protein levels were increased after ectopic expression FOXA1 for 48 h and restored by transfection of HCT116 cells with CYR61 siRNA for 48 h. f FOXA1 ectopic expression increased HCT116 cell migration, while CYR61 knockdown restored HCT116 cells migration. g CYR61 protein levels were decreased after transfection with FOXA1 siRNA for 72 h and restored by ectopic expression of CYR61 for 48 h in RKO cells. h FOXA1 Knockdown decreased RKO cell migration, while ectopic expression of CYR61 partially restored RKO cell migration. i CYR61 protein levels were increased after LV-Cherry-neomycin/FOXA1 lentivirus (CV572-mFOXA1) transduction and restored by transduction of HCT116 cells with CYR61 shRNA (GV344-shCYR61). j Representative images of metastatic signal detected by IVIS in NOD SCID mice 28 days after tail vein injection (n = 5/group). Quantification of the luciferase signal by IVIS. Significance for all data was determined by the independent samples t-test. Data are shown as mean ± S.D., n = 3. *P < 0.05, **P < 0.01