Fig. 5

GPAA1 facilitates in vivo tumour growth, which can be inhibited by trastuzumab. a Tumours isolated from mice injected subcutaneously with AGS cells (sh-NC, sh-GPAA1–1, sh-GPAA1–2). b-c Tumour weights and tumour volumes in the GPAA1-Ctrl and GPAA1-downregulation groups. d IHC staining of GPAA1 and Ki-67 in sh-Ctrl and sh-GPAA1 tumours (scale bar: 50 μm). e Tumours obtained from mice injected subcutaneously with HGC-27 cells with either over- or normal expression of GPAA1 and treated with vehicle or trastuzumab. f-g Tumour weights and tumour volumes in each group. h IHC staining of GPAA1, p-AKT and Ki-67 in each group (scale bar: 50 μm). *P < 0.05, **P < 0.01, ***P < 0.001