Table 1 Sequences of primers used for real-time PCR
Primer | Sequence 5′-3′ |
|---|---|
GPAA1 Forward | CTCCCGCTTCGTCTCCATC |
GPAA1 Reverse | CACTGGCAGGACATAGAGGG |
P21 Forward | CGATGGAACTTCGACTTTGTCA |
P21 Reverse | GCACAAGGGTACAAGACAGTG |
P27 Forward | ATCACAAACCCCTAGAGGGCA |
P27 Reverse | GGGTCTGTAGTAGAACTCGGG |
CyclinB1 Forward | AATAAGGCGAAGATCAACATGGC |
CyclinB1 Reverse | TTTGTTACCAATGTCCCCAAGAG |
CyclinD1 Forward | CAATGACCCCGCACGATTTC |
CyclinD1 Reverse | CATGGAGGGCGGATTGGAA |
CDK4 Forward | TCAGCACAGTTCGTGAGGTG |
CDK4 Reverse | GTCCATCAGCCGGACAACAT |
CDK6 Forward | CCAGATGGCTCTAACCTCAGT |
CDK6 Reverse | AACTTCCACGAAAAAGAGGCTT |
GAPDH Forward | CTGGGCTACACTGAGCACC |
GAPDH Reverse | AAGTGGTCGTTGAGGGCAATG |