Fig. 2
Effect of PYK2 knockdown on cell migration of the HER2+/ER−/PR- breast cancer cell lines SkBr3 and MDA-MB-453. a, b Immunoblot images representing PYK2 expression SkBr3 and MDA-MB-453 control (pLKO.1 empty vector) and PYK2 knockdown cells. N = 3 (3 replicates). c Wound healing assay (scratch assay) using SkBr3 control and PYK2 knockdown metformin treated and untreated cells, and the corresponding data quantifying gap closure at time points 0 and 48 h following scratching. Anova ****P = < 0.0001, ***P = 0.0003 (Empty vector vs. PYK2 shRNA1), ***P = 0.0004 (Empty vector vs. PYK2 shRNA2). d Data quantifying gap closure at time points 0 and 48 h following scratching Wound healing assay (scratch assay) using MDA-MB-453 control and PYK2 knockdown metformin treated and untreated cells. Anova ****P = < 0.0001. N = 3 (2 replicates)