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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: PYK2 promotes HER2-positive breast cancer invasion

Fig. 3

Effect of PYK2 knockdown on cell proliferation and invasion of the HER2+/ER−/PR- breast cancer cell lines SkBr3 and MDA-MB-453. a Cell invasion assay using SkBr3 cells metformin treated and untreated and the corresponding data quantifying of number of invading cells (48 h with or without treatment). Anova ****P = < 0.0001, **P = 0.0025 (Treated empty vector vs. treated PYK2 shRNA1), ***P = 0.0032 (Treated empty vector vs. treated PYK2 shRNA2). N = 3 (2 replicates). b Cell invasion assay using MDA-MB-453 metformin treated and untreated cells, and the corresponding data quantifying number of invading cells (48 h with or without treatment). Anova ****P = < 0.0001, **P = 0.0032 (Empty vector vs. treated empty vector), **P = 0.0030 (Empty vector vs. PYK2 shRNA1), **P = 0.0017 (Empty vector vs. PYK2 shRNA1), ***P = 0.0005 (Treated empty vector vs. treated PYK2 shRNA2), ***P = 0.0005 (Treated empty vector vs. treated PYK2 shRNA1). N = 3 (2 replicates). c Cell proliferation assay using SkBr3 metformin treated and untreated cells, and the corresponding data quantified as cell index (48 h with or without treatment). Anova, ***P = 0.0001 (Empty vector vs. PYK2 shRNA1), ***P = 0.0004 (Empty vector vs. PYK2 shRNA2), ***P = 0.0002 (Treated empty vector vs. treated PYK2 shRNA1), **P = 0.0003 (Treated empty vector vs. treated PYK2 shRNA2). N = 3 (2 replicates). d Cell proliferation assay using MDA-MB-453 metformin treated and untreated cells, and the corresponding data quantified as cell index (48 h with or without treatment). Anova, **P = 0.0034 (Empty vector vs. treated empty vector), **P = 0.0010 (Empty vector vs. PYK2 shRNA1), ***P = 0.0003 (Empty vector vs. PYK2 shRNA2), **P = 0.0060 (Treated empty vector vs. treated PYK2 shRNA1), **P = 0.0022 (Treated empty vector vs. treated PYK2 shRNA1), ***P = 0.0007 (Untreated PYK2 shRNA1vs. treated PYK2 shRNA1), ***P = 0.0004 (Untreated PYK2 shRNA2vs. treated PYK2 shRNA2). N = 3 (2 replicates). e Schematic representation of the dual role of PYK2 in proliferation, migration and invasion of HER2+/ER−/PR- breast cancer in response to metformin

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