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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The m6A-suppressed P2RX6 activation promotes renal cancer cells migration and invasion through ATP-induced Ca2+ influx modulating ERK1/2 phosphorylation and MMP9 signaling pathway

Fig. 4

The ATP-P2RX6-Ca2+ signaling mediated the MAPK family ERK1/2 activation to promote RCC migration/invasion. a qRT-PCR assay for screening some metastasis genes after manipulating P2RX6 in SN12-PM6 and 786-O cells. b Correlation analysis for P2RX6 and MMP9 from TCGA database. c Immunofluorescence showed that ATP could stimulate the expression of MMP9. d WB of MMP9 using P2RX6-shRNA. e WB of MMP9 using Ca2+ inhibitor verapamil (2uM). f WB of MMP9 using ERK1/2 inhibitor SCH772984 (0.1uM). WB to show MMP9 Knockdown efficiency in J82 and 5637 cells. g WB of MMP9 using MMP9-shRNA. h Migration assay using sh-MMP9 in 786-O and OS-RC-2 cells treated with ATP. i Transwell assay after sh-MMP9 in 786-O and OS-RC-2 cells treated with ATP. Scale bar, 200 μm. j Quantitative analysis for Fig. 4i. k MMP9 gene information on http://www.oncolnc.org/ and Kaplan-Meier analysis for MMP9 mRNA expression in RCC patients (***P = 0.000852). N.S. = not significant. * P < 0.05, ** P < 0.01, *** P < 0.001

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