Fig. 5
The m6A modification is decreased in RCC and P2RX6 can be regulated by METTL14. a P2RX6 potential m6A sites distribution. b The m6A contents of total RNAs in tumor tissues paired with para-tumor tissues (n = 7). c The mRNA levels of m6A modification associated genes in 7 pairs of RCC tumor and para-tumor tissues. d Expression of METTL14 in TCGA KIRC. e Kaplan-Meier analysis for METTL14 expression level on KIRC patients’ OS. f Correlation between METTL14 and P2RX6 from TCGA database. g Correlation between METTL14 and P2RX6 from website R2. h Validation of METTL14 mRNA and protein level knocking-down efficiency in 786-O cells. i The m6A contents of total RNAs in 786-O. Dot blot assay analyzed the Poly(A)+ RNAs isolated from pLKO and sh-M14 group cells. RNAs were equally loaded by two fold serial dilution. Methylene blue was used to detect equal mRNA loading in two groups. j P2RX6 mRNA and protein expression level after knocking-down METTL14. k Validation of METTL14 mRNA and protein level knocking-down efficiency in OS-RC-2 cells. l The m6A contents of total RNAs in SN12-PM6 cell line. m P2RX6 mRNA and protein expression level after over-expression METTL14. M14 = METTL14, N.S. = not significant. * P < 0.05, ** P < 0.01, *** P < 0.001