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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Inhibition of ERK1/2 in cancer-associated pancreatic stellate cells suppresses cancer–stromal interaction and metastasis

Fig. 2

Expression of p-ERK1/2 in PDAC tissues and pancreatic cell lines. a p-ERK1/2 expression was detected in both pancreatic cancer cells and stromal cells. Scale bars =100 μm. b p-ERK1/2 expression was detected in KPC mice cancer cells (a) and stromal cells (b) of pancreatic primary tumor, and liver metastases (c). Scale bars =100 μm. c Western blot of ERK1/2, p-ERK1/2, and α-SMA levels in pancreatic cells. d Western blot of ERK1/2 and p-ERK1/2 levels in PCCs, alone or after co-culture with PSCs. PCCs were seeded in 24-well plates while PSCs were seeded in the upper transwell chamber with 3-μm pore size. e SUIT-2, (f) AsPC-1 Migration and invasion assays were performed for 18 and 36 h, respectively. PSCs were seeded in 24-well plates while PCCs were seeded in the upper transwell chamber of 8-μm pore size. Graphs show numbers of cells calculated from five fields. Scale bars =100 μm. *P < 0.05, **P < 0.01

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