Fig. 5

Inhibition of ERK1/2 suppressed PCC-PSC interaction by preferentially targeting PSC. a In indirect co-culture experiments, first PSCs were seeded, and 24 h later, medium was replaced and transwell chambers (8-μm pores; Becton Dickinson) were placed in 24-well dishes, and then PCCs were seeded into the transwell chambers. After incubation for the indicated time, migration and invasion were evaluated by counting the cells that had invaded to the lower chamber. SCH772984 dose was used IC50 value of PSC2 cells, 89 nM. b Migration and invasion assays were performed for 18 and 36 h, respectively. Graphs show numbers of cells calculated from five fields. Scale bars =100 μm. *P < 0.05, **P < 0.01. c Viability of SLMS cells co-cultured with (d) PSC1 or (e) PSC2 cells after DMSO or SCH772984 treatment; (g) Viability of SLMA cells co-cultured with (h) PSC1 or (i) PSC2 cells after DMSO or SCH772984 treatment was determined by CellTiter-Glo luminescent cell viability assay. SCH772984 dose was used IC50 value of PSC1, 321 nM; and PSC2, 90 nM. *P < 0.05. Columns, mean fold changes of three experiments done in triplicate