Fig. 6

Dual treatment of SCH772984 and CQ decreased cell viability and induced senescence of PSCs. a Western blot of fibronectin, α-SMA, LC3-II, Akt and p-Akt levels of PSCs after treatment with ERK inhibitor. b Microphotograph of PSCs after indicated agent treatment. Scale bars = 100 μm. c Cell viability of PSCs after indicated agent treatment. d qRT-PCR shows mRNA expression of α-SMA, Collagen Type I, p15 and p16. *P < 0.05, **P < 0.01, ***P < 0.001. e β-galactosidase staining of PSCs after indicated agent treatment. Bottom: graphs show the quantification of β-gal-positive cells calculated from five fields. Scale bars =100 μm. *P < 0.05. e Migration and invasion assays were performed for 18 and 36 h, respectively. Graphs show numbers of cells calculated from five fields. Scale bars =100 μm. *P < 0.05. f Cell viability of PCCs after indicated agent treatment. Columns, mean fold changes in three experiments done in triplicate. g Western blot of indicated protein levels in PCCs after indicated treatment