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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Inhibition of ERK1/2 in cancer-associated pancreatic stellate cells suppresses cancer–stromal interaction and metastasis

Fig. 7

Dual treatment of SCH772984 and CQ decreased liver metastasis in xenograft organoid model with PSC co-transplantation. a Microphotograph of KPC mouse-derived cancer organoid. Scale bars =100 μm. b Scheme of xenograft experiment. Female nude mice were intrasplenic transplanted with cancer organoids with PSCs and randomized divided into four groups (n = 5/group). One week after implantation, mice were dosed once daily with vehicle, SCH772984 (25 mg/kg), Chloroquine (50 mg/kg), or dual treatment of each group for 13 days. Dosing occurred from day 14 to day 26. At day 27, mice were sacrificed and liver metastases were harvested. c Gross pathology showed significantly reduced liver metastasis formation after dual treatment of SCH772984 with chloroquine. d Liver metastasis nodules were significantly reduced in samples treated with SCH772984 or CQ, or both. *P < 0.05, ***P < 0.001. e Tumor weight and (f) volume were significantly decreased only in the dual-treatment group. *P < 0.05, ***P < 0.001. g Immunohistochemical staining shows decreased p-ERK1/2 expression in SCH772984 group, and decreased α-SMA expression in CQ group; The dual-treated group showed significant reductions of p-ERK1/2, α-SMA, Ki67 and collagen fibers. Corresponding rectangles indicated a-SMA-positive PSCs. Right: quantification of gene expression from five fields. Scale bars =100 μm. *P < 0.05, **P < 0.01

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