Fig. 6

Additive effects of tumor cell lipid loading and systemic lipids on angiogenesis, macrophage infiltration, and survival in glioma. a, GL261 mouse GBM cells were pre-incubated in hypoxia without (LD⁻) or with extracellular lipid (LD⁺) for 48 h, and then injected (100.000 cells/animal) into the brains of C57BL/6 mice on chow diet (WT) or hyperlipidemic ApoE^−/− mice on high fat diet (HFD). b, Kaplan-Meier survival curves (N = 7 animals per group). Shown are all groups (left panel), or comparing effect of tumor cell lipid preloading in the hyperlipidemic background (mid panel) or the effect of systemic lipid level background in preloaded tumor cells (right panel). c, e, and g, Immunofluorescence staining of F4/80 (red) (c), αSMA (green) (e) and vWF endothelial cell marker (red) (g) and corresponding quantifications relative to nuclei area (d, f, and h) (from 4 representative animals per group, 9–18 fields per animal for each marker). Scale bars, 150 μm