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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Efficient disruption of bcr-abl gene by CRISPR RNA-guided FokI nucleases depresses the oncogenesis of chronic myeloid leukemia cells

Fig. 1

Schematic diagram of dimeric RFNs target bcr-abl to induce gene modification. a Dimeric FokI-dCas9 proteins target to DNA half-site sequences with PAM out orientation by pairs of gRNAs to cleave the intervening spacer sequence of c-abl exon 2. The gRNA-15, gRNA-16, gRNA-17, and gRNA-half showed the target site in c-abl exon 2. b The structure of FokI-dCas9 nucleases expression plasmid

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