Fig. 1

Schematic diagram of dimeric RFNs target bcr-abl to induce gene modification. a Dimeric FokI-dCas9 proteins target to DNA half-site sequences with PAM out orientation by pairs of gRNAs to cleave the intervening spacer sequence of c-abl exon 2. The gRNA-15, gRNA-16, gRNA-17, and gRNA-half showed the target site in c-abl exon 2. b The structure of FokI-dCas9 nucleases expression plasmid