Skip to main content


Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Efficient disruption of bcr-abl gene by CRISPR RNA-guided FokI nucleases depresses the oncogenesis of chronic myeloid leukemia cells

Fig. 4

RFNs suppress viability and induce apoptosis of imatinib sensitive and resistant cells. Cells were transfected with gRNA-17 plus donor, RFNs-half plus donor, RFNs plus donor, respectively. a Evaluation of cell proliferative capacity by CCK-8 assay. b, c The capacity of colony formation was assessed by colony-forming assay. d Cell nucleuses were stained by DAPI to detect the morphologic changes caused by apoptosis. The white arrows pointed out the typical apoptotic cells. e Satistical analysis result of apoptotic cells tested by flow cytometry. f The activation of apoptotic pathway was investigated by western blot. The results are presented as the means ± SD. One-way ANOVA analysis was used to compare treated groups with control group, p < 0.01 (**) and p < 0.001 (***)

Back to article page