Fig. 5

The proliferation was suppressed and apoptosis was induced by RFNs in CML stem/progenitor cells. CML CD34⁺ cells were transfected with gRNA-17 plus donor, and RFNs plus donor. a The evaluation of modification efficiency of RFNs on bcr-abl by NotI digestion. b Cell viability of CML stem/progenitor cells was tested by CCK-8 assay. c, d Percentage of apoptotic CML stem/progenitor cells was tested by flow cytometry. All results are exhibited as the mean ± SD, p < 0 .01 (**) and p < 0.001 (***)