Fig. 13

In vivo experiments (a) The nude mice were divided into five groups (1–7) by different treatment, 1: PBS, 2: Nek2B-siRNA control, 3:β-catenin control, 4:paclitaxel, 5: Nek2B siRNA, 6: Nek2B siRNA combining with paclitaxel, 7: Nek2B siRNA and paclitaxel combining with β-catenin. Total RNA extracts from the tumors which were transfacted with Nek2B-siRNA in Hs578T cells (b) and Nek2B plasmid in MDA-MB-231 cells (c) were examined for expression levels of Nek2B, β-catenin and downstream target genes. *P<0.05; **P<0.01