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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Nek2B activates the wnt pathway and promotes triple-negative breast cancer chemothezrapy-resistance by stabilizing β-catenin

Fig. 8

Hs578T (a) and BT20 (b) cells were transfected with Nek2B siRNA, Nek2B and β-catenin mRNA levels were analyzed by qRT-PCR; (c) Western blot analysis was used to detect the Nek2B and p-β-catenin protein expression in Hs578T cells after transfected with Nek2B siRNA. Actin is shown as loading control; MDA-MB-231(d) and MDA-MB-468 (e) cells were transfected with Nek2B plasmid, Nek2B and β-catenin mRNA levels were analyzed by qRT-PCR; (f) Western blot analysis was used to detect the Nek2B and p-β-catenin protein expression in MDA-MB-231 cells after transfected with Nek2B plasmid; Nucleoplasmic separation technique was used to detect the expression of β-catenin in nucleus and cytoplasm after Hs578T cells were transfected with Nek2B siRNA (g) and MDA-MB-231 cells were transfected with Nek2B plasmid (h). *P<0.05; **P<0.01

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