Skip to main content

Advertisement

Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Inhibition of RNA polymerase III transcription by Triptolide attenuates colorectal tumorigenesis

Fig. 6

Triptolide represses TFIIIB formation at Pol III promoters. a RKO cells were incubated with 40 nM triptolide for the indicated time. The mRNA level of TBP, Brf1, Bdp1, and POLR3D was measured by real-time qPCR. b RKO cells were incubated with triptolide at the indicated concentration for 0.5 h. The mRNA level of TBP, Brf1, Bdp1, and POLR3D was measured by real-time qPCR. c RKO cells expressing FLAG-tagged TBP, Brf1, or POLR3D were treated with or without 40 nM triptolide for 0.5 h. Chromatin immunoprecipitation was carried out using FLAG antibody and the immunoprecipitates were subjected to qPCR

Back to article page