Fig. 1

MALAT1 is up-regulated in GBC tissues and cells. a, the relative expression of MALAT1 in normal gallbladder tissues (n = 16) and GBC tissues (n = 48). * p < 0.05 vs. the normal gallbladder tissue. b, the correlation between the expression of MALAT1 and DFS and OS of patients analyzed by the Kaplan-Meier method. c, the relative expression of MALAT1 in GBC cells (GBC-SD, SGC-996, NOZ and OCUG1) and HGBECs determined by RT-qPCR. d and e, the relative expression of MALAT1 in GBC-SD and SGC-996 cells in the presence of MALAT1 silencing. * p < 0.05 vs. the HGBEC cell line or cells manipulated with sh-NC. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test. The experiment was repeated 3 times. MALAT1, metastasis associated lung adenocarcinoma transcript 1; GBC, gallbladder cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; NC, negative control; HGBECs, human gallbladder epithelial cells; DFS, disease-free survival; OS, overall survival