Fig. 2

MALAT1 silencing restrains the proliferation, migration and invasion of GBC cells while promotes the apoptosis and senescence. a, the proliferation of GBC-SD and SGC-996 cells detected by EdU assay (200 ×). b, the protein expression of Ki67 and PCNA relative to β-actin in GBC-SD and SGC-996 cells determined by Western blot analysis. c, the migration of GBC-SD and SGC-996 cells detected by scratch test. d, the invasion of GBC-SD and SGC-996 cells detected by Transwell assay (200 ×). e, the senescence of GBC-SD and SGC-996 cells detected by SA-β-gal staining (400 ×). * p < 0.05 vs. the GBC-SD or SGC-996 cells transfected with sh-NC. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test. The experiment was repeated 3 times. MALAT1, metastasis associated lung adenocarcinoma transcript 1; GBC, gallbladder cancer; EdU, 5-ethynyl-2′-deoxyuridine; PCNA, proliferating cell nuclear antigen; SA-β-gal, senescence-associated β-galactosidase; NC, negative control