Fig. 4

ABI3BP over-expression restrains the proliferation and metastasis of GBC cells while contributes to senescence. a and b, the proliferation of GBC-SD cells detected by EdU assay (200 ×). c and d, the protein expression of Ki67 and PCNA relative to β-actin in GBC-SD cells determined by Western blot analysis. e, the migration of GBC-SD cells detected by scratch test. f, the invasion of GBC-SD cells detected by Transwell assay (200 ×). g, the senescence of GBC-SD cells detected by SA-β-gal staining (400 ×). * p < 0.05 vs. the GBC cells manipulated with vector-NC. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test. The experiment was repeated 3 times. ABI3BP, ABI family member 3 binding protein; GBC, gallbladder cancer; EdU, 5-ethynyl-2′-deoxyuridine; PCNA, proliferating cell nuclear antigen; SA-β-gal, senescence-associated β-galactosidase; NC, negative control