Fig. 5

MALAT1 down-regulates the expression of ABI3BP through EZH2 recruitment in the promoter region of ABI3BP. a, the subcellular localization of MALAT1 predicted on lncATLAS website. b, Blast comparison between sequences of MALAT1 and ABI3BP promoter. c, the subcellular localization of MALAT1 detected by FISH assay (400 ×). d, the enrichment of EZH2 by MALAT1 detected by RIP assay. e, the relative expression of EZH2 relative to β-actin detected by RNA pull down. f, EZH2 enrichment in the ABI3BP promoter region detected by ChIP assay. g, the relative expression of ABI3BP determined by RT-qPCR. * p < 0.05 vs. the cells treated with sh-NC, Bio-probe-NC or DMSO. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test between two groups and by one-way analysis of variance among multiple groups, followed by Turkey’s post-hoc test. The experiment was repeated 3 times. MALAT1, metastasis associated lung adenocarcinoma transcript 1; ABI3BP, ABI family member 3 binding protein; EZH2, enhancer of zeste homolog 2; FISH, fluorescence in situ hybridization; RIP, RNA immunoprecipitation; ChIP, chromatin immunoprecipitation; RT-qPCR, reverse transcription quantitative polymerase chain reaction; NC, negative control; DMSO, dimethyl sulfoxide