Fig. 3

miR-126-3p replacement induces G0/G1 arrest in dabrafenib-resistant cells and decreases the levels of phosphorylated ERK1/2 and/or AKT. a Melanoma cells were transiently transfected with 50 nM pre-miR-126-3p or pre-miR-CTRL and 72 h later removed from continuous culture and analyzed for apoptosis induction and cell cycle perturbations. Each value represents the arithmetic mean of four (A375R) or three (SK-Mel28R) independent experiments. Bars, SEM. ^**P<0.01 and ^*P<0.05, pre-miR-126-3p vs matched pre-miR-CTRL. b Flow cytometry profiles relative to a representative experiment. c Melanoma cells were transfected as described in (a) and 48 h later cell lysates were analyzed by immunoblotting using antibodies against pospho-ERK1/2 (Thr202/Tyr204) (p-ERK1/2), ERK1/2, phospho-AKT (Ser473) (p-AKT), AKT or β-tubulin. The results are representative of two independent experiments giving comparable results.