Fig. 4

miR-126-3p replacement increases response of drug-resistant cells to dabrafenib. a, b Melanoma cells were transiently transfected with 50 nM pre-miR-126-3p or pre-miR-CTRL and 24 h later incubated with graded concentrations of dabrafenib (DAB) or with DMSO alone. After five days of culture, proliferation was assessed by the MTT assay. Data are expressed in terms of absorbance at 595 nM. Each value represents the arithmetic mean of four (A375R) or five (SK-Mel28R) independent experiments performed with triplicate samples. Bars, SEM. ^##P<0.01 and ^#P<0.05, pre-miR-126-3p vs matched pre-miR-CTRL; ^**P<0.01 and ^*P<0.05 pre-miR-126-3p/DAB vs pre-miR-126-3p/DMSO; ^§P<0.05 pre-miR-CTRL/DAB vs pre-miR-CTRL/DMSO.