Fig. 9

ADAM9 silencing delays the development of resistance to dabrafenib. A375 cells were seeded into 96-well plates and every eight days transfected with 50 nM siADAM9 or siCTRL and treated with 100 nM dabrafenib (DAB) or DMSO. Cell cultures were photographed and processed for quantitative analysis of proliferation on day 0 (i.e after the first transfection), 8, 16 and 24. Images from a representative experiment are shown. b Quantitative analysis of proliferation of cell cultures described in (a). Crystal violet was solubilized and absorbance was read at 595 nm. Each value represents the arithmetic mean of three independent experiments performed with triplicate cultures. Bars, SEM. ^**P<0.01, siADAM9 vs matched siCTRL; ^§§P<0.01, siCTRL/DAB/Day 8 vs siCTRL/DMSO/Day 8; ^††P<0.01, siADAM9/DAB/Day 8 vs siADAM9/DMSO/Day8; ^##P<0.01, siCTRL/DAB/Day 16 vs siCTRL/DAB/Day 8; ^⁋⁋P<0.01, siCTRL/DAB/Day 24 vs siCTRL/DAB/Day 16.