Fig. 5

Reducing lncRNA DLX6-AS1 suppresses the STAT3 signaling pathway via the upregulation of CADM1. a, nuclear import of STAT3 detected by immunofluorescence staining, scale bar = 25 μm; b, CADM1 expression in LCSCs isolated from Huh7 and HepG2 determined by RT-qPCR; c-e, CADM1 protein levels and phosphorylation levels of STAT3 in LCSCs isolated from Huh7 and HepG2 determined by western blot analysis; ^*, p < 0.05, vs. the blank group; the statistical data were expressed as mean value of standard error and analyzed by one-way ANOVA; the experiment was conducted 3 times; CADM1, cell adhesion molecule 1; STAT3, signal transducer and activator of transcription 3; RT-qPCR, reverse transcription quantitative polymerase chain reaction; ANOVA, analysis of variance; lncRNA, long non-coding RNA; DLX6-AS1, DLX6 antisense RNA 1