Fig. 6

Reduced lncRNA DLX6-AS1 inhibits spheroid formation ability, colony formation ability, and proliferation ability of LCSCs via the inhibition of CADM1–mediated STAT3 signaling pathway. a, mRNA levels of LCSCs markers determined by RT-qPCR; b and c, protein levels of LCSCs markers determined by western blot analysis; d, spheroid formation ability of LCSCs detected by spheroid formation assay, scale bar = 100 μm; e, colony formation ability of LCSCs detected by colony formation assay, scar bar = 50 μm; f, proliferation ability of LCSCs determined by EdU staining, scale bar = 50 μm; ^*, p < 0.05, vs. the blank group; the statistical data were expressed as mean value of standard error and analyzed by one-way ANOVA; the experiment was conducted 3 times; CADM1, cell adhesion molecule 1; STAT3, signal transducer and activator of transcription 3; RT-qPCR, reverse transcription quantitative polymerase chain reaction; ANOVA, analysis of variance; LCSCs, liver cancer stem cells; lncRNA, long non-coding RNA; DLX6-AS1, DLX6 antisense RNA 1; CD133, prominin-1; CD13, aminopeptidase N