Fig. 1

Isovitexin inhibits carcinogenicity and stemness in HCSLCs. a Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies used as a loading control; b and c Formed spheres and colonies (Scale bar, 200 μm); d Immunoblotting for CD133, CD44 and ALDH1, Bmi1, Nanog and Oct4; e CD133⁺ cell population in MHCC97H cells and HCSLCs. f Decreased the amounts of MnSOD and FoxM1 in HCSLCs from MHCC97H cells following treatment with isovitexin (ISOV: 5.0, 10.0 and 20.0 μM). Isovitexin induced reduction of spheroid formation (g, Scale bar, 200 μm), colony formation (h, Scale bar, 200 μm), and protein amounts of CD133, CD44, ALDH1 and Bmi1, Nanog and Oct4 (i), CD133⁺ cell population (j) in HCSLCs