Fig. 3

Effects of isovitexin combined with MnSOD shRNA or cDNA on carcinogenicity and stemness in HCSLCs or MHCC97H cells. HCSLCs were transduced with Ad-GFP and Ad-shMnSOD, respectively, incubated with or without isovitexin (ISOV; 10 μM). a Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies as a loading control; b and c Formed spheres and colonies (Scale bar, 200 μm); d Immunoblotting for CD133, CD44, ALDH1, Bmi1, Nanog and Oct4; e The relative luciferase activity of FOXM1 promoter fragment in HCSLCs transduced with Ad-GFP and Ad-shMnSOD, respectively, in the absence or presence of isovitexin. MHCC97H cells were transduced with Ad-GFP and Ad-MnSOD, respectively, and incubated with or without isovitexin. f Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies as a loading control; g and h Formed spheres and colonies (Scale bar, 200 μm); i Immunoblotting for CD133, CD44, ALDH1, Bmi1, Nanog and Oct4; j The relative luciferase activity of FOXM1 promoter fragment in MHCC97H cells transduced with Ad-GFP and Ad-MnSOD, respectively, cultured in the absence or presence of isovitexin