Fig. 4

Effects of FoxM1 shRNA or cDNA transduction on carcinogenicity and stemness in HCSLCs or MHCC97H cells. HCSLCs were transduced with Ad-GFP or Ad-shFoxM1. a Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies as a loading control; b and c Formed spheres and colonies (Scale bar, 200 μm); d Immunoblotting for CD133, CD44, ALDH1, Bmi1, Nanog and Oct4 in HCSLCs untreated (Mock), and transduced with Ad-GFP and Ad-shFoxM1, respectively. MHCC97H cells were transduced with Ad-GFP and Ad-FoxM1, respectively. e Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies as a loading control; f and g Formed spheres and colonies (Scale bar, 200 μm); h Immunoblotting for CD133, CD44, ALDH1, Bmi1, Nanog and Oct4in MHCC97H cells untreated (Mock), and transduced with Ad-GFP and Ad-FoxM1, respectively