Fig. 2

Gene expression analyses in tumorspheres from ovarian cancer cell lines. a Gene expression analyses by RT-qPCR of up- and down-regulated genes in tumorspheres and total adherent cell culture (total culture) samples from SKOV3 and OVCAR8 ovarian cancer cell lines. b Gene expression analyses by RT-qPCR of C-KIT, CD133, NANOG, CXCR4 and ABCG2 in tumorspheres and total culture samples from SKOV3 and OVCAR8 ovarian cancer cell lines. For (a) and (b), normalized expression values of tumorspheres compared to total culture samples (line at value 1) are shown. The average and SD of three independent experiments are shown. Statistical significance was assessed using the one-sample t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001