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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis

Fig. 2

Targeting the Snail/Slug-mediated epithelial-to-mesenchymal transition (EMT) process by apigenin (API) results in suppression of the invasion of prostate cancer cells. a, b PC-3 M and DU145 cells were treated with API at 40 μM for different durations (a), or at an increasing dose for 24 h (b), and EMT-related transcription factors (Snail, Slug, and Twist) were evaluated by Western blotting. c After API treatment for 24 h, protein expression levels of EMT markers in PC-3 M and DU145 cells were determined by Western blotting. d PC-3 M and DU145 cells were transfected with either pLEX-Snail, pCIneo-Slug, or their respective vector controls for 24 h, and then expression levels of Slug (left panel) and Snail (right panel) were examined by Western blotting. e, f The vector control or overexpressing cells were incubated with 40 μM API for 48 h, and cell invasive abilities were measured by a transwell assay. Representative photographs of invasive cells (left) and quantification of these cells (right) are shown. Data are presented as the mean ± SD of three independent experiments, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. untreated cells and # p < 0.05, ## p < 0.01, ### p < 0.001 vs. 40 μM API-treated cells

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