Fig. 1

NEO212 exerts stronger cytotoxicity than its individual constituents in vitro. a A2780, SK-O-V3 and OVCAR-3 cells were treated with 0, 25, 50, 100, 150, 200, 300 μM TMZ, POH, TMZ + POH and NEO212 respectively for 48 h, DMSO acted as the control, and then subjected to MTT assay. Absorbance value was calculated and standardized to DMSO group. Three independent experiments were performed. b The above cells were treated with 100 μM TMZ, POH, TMZ + POH and NEO212 or DMSO for 48 h, respectively, then drugs were withdrawn and cells grew in normal culture for 14 days, DMSO acted as the control, and subjected to cell colony formation assay Statistical differences of cell colony formation assay were calculated and presented as mean ± SD. c A2780, SK-O-V3 and OVCAR-3 cells were treated with 100 μM TMZ, POH, TMZ + POH, NEO212 or DMSO respectively for 48 h, and the cell cycle distributions were analyzed. The results shown are means ± SD; *p < 0.05; **p < 0.01; ***p < 0.001