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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: NEO212 induces mitochondrial apoptosis and impairs autophagy flux in ovarian cancer

Fig. 3

NEO212 induces DNA damage and mitochondrial apoptosis. a-c Cells were treated with 100 μM TMZ, POH, TMZ + POH, NEO212 or DMSO respectively for 48 h. a Western blot analysis demonstrated p-ATM, p-CHEK1, p-CHEK2, p-H2AFX and ACTB expression in above drug-treated A2780 and SK-O-V3 cells. b Cells were subjected to apoptosis assay using Annexin-V &PI staining. c Lysates from above drug-treated A2780 and SK-O-V3 cells were subjected to western blot using following antibodies against cleaved CASP9, pro-CASP3, cleaved CASP3, Cytochrome C (Cyto C), BAX and ACTB. d A2780 and SK-O-V3 cells were treated with 100 μM NEO212 for 48 h with or without Ac-LEHD or Z-VAD, and then apoptosis assay using Annexin-V &PI staining. The results shown are means ± SD; *p < 0.05; **p < 0.01

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