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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway

Fig. 4

PLEK2 suppressed EGFR degradation. a Protein levels of EGFR in PLEK2 knockdown and overexpression cells were detected by western blot. b, c Alterations of EGFR degradation in PLEK2 knockdown cells in response to 100 nM CHX treatment for the indicated time were detected by western blot. d EGFR expression in PLEK2 knockdown cells in response to 10 μM MG132 treatment for 6 h were detected by western blot. e The ubiquitination level of EGFR with increasing ectopic PLEK2 expression were detected by co-IP. f Co-IP of overexpressed PLEK2, c-CBL and EGFR in 293 T cells. g Co-IP of Flag-c-CBL and EGFR, together with increasing amount of Myc-PLEK2 in 293 T cells. All experiments were repeated at least three times, and data were analyzed using student’s t-test. * P < 0.05. Error bars indicate SEM

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