Fig. 5

Oncogenic effects of PLEK2 depended on EGFR pathways. a, b Protein levels of EGFR downstream signaling in indicated GBC cell lines with either PLEK2 knockdown or overexpression were detected by western blot. c Migration and invasion ability of PLEK2 knockdown NOZ cells with increasing ectopic EGFR expression were measured. d Protein levels of EGFR downstream signaling in PLEK2 knockdown cells with increasing ectopic EGFR expression were detected by western blot. e Migration and invasion ability of PLEK2 overexpression NOZ cells with EGFR inhibitor erlotinib treatment were measured. f Protein levels of EGFR downstream signaling in PLEK2 overexpression cells with 1 mmol/L erlotinib treatment for 12 h were detected. All experiments were repeated at least three times, and data were analyzed using student’s t-test. * P < 0.05. Error bars indicate SEM