Fig. 1

AJAP1 was low expressed in breast cancer and inhibited the proliferation of breast cells. a Dataset from Oncomine and GOBO on AJAP1 in tumors tissues versus non-tumor tissues (n = 59), different subtypes of breast cancer(n = 1881) and grades(n = 1411). b AJAP1 protein and mRNA levels in 25 pairs of tumor tissues versus non-normal tissue. c Representative IHC image of AJAP1 expression location in different stages of breast cancer (normal lobules, Ductal carcinoma in situ, invasive cancer). d overall survival of AJAP1 in 283 cases of breast cancer. e AJAP1 protein levels in breast cancer cell lines. f confirmation of upregulation and downregulation of AJAP1 expression by Western blot and qRT-PCR (g-k) effects of AJAP1 knockdown and overexpressed on in vitro proliferation (g, i), invade (h) migration(j) and cell cycle progression (k) Data were shown as mean ± SD *p < 0.05; **p < 0.01; ***p < 0.001