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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Houttuynia cordata Thunb. and its bioactive compound 2-undecanone significantly suppress benzo(a)pyrene-induced lung tumorigenesis by activating the Nrf2-HO-1/NQO-1 signaling pathway

Fig. 4

H. cordata and 2-undecanone protect BEAS-2B cells and A/J mice from B[a]P-induced DNA damage. a Representative comet tail images (scale bar: 50 μm) showing the DNA damage response after treatment with vehicle, B[a]P (5 μM) alone, B[a]P (5 μM) plus H.cordata (12.5, 25, or 50 mg/mL), or B[a]P (5 μM) plus 2-undecanone (25, 50, or 100 μM) for 48 h. The protein levels of the DNA damage marker p-H2A.X were detected in the cells after the same treatment regimens with H. cordata (b) or 2-undecanone (c) by Western blot analysis. d Representative confocal images (scale bar: 50 μm) of double-stained cells subjected to the described treatments and stained for p-H2A.X (red) and with DAPI (blue). e The protein levels of p-H2A.X were detected in mice after treatment with H.cordata (25 or 50 g/kg) (e) or 2-undecanone (100 or 200 mg/kg) (F) by Western blot analysis. The data represent the mean ± SD (n = 3). ###p < 0.001 compared with the control cells (given water); *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the model cells (given B[a]P)

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