Fig. 5

H. cordata and 2-undecanone protect BEAS-2B cells and A/J mice from B[a]P-induced inflammation. The protein levels were measured by using Western blot analysis. a and b The protein levels of IL-1β in the culture supernatants were detected after treatment with vehicle, B[a]P (5 μM) alone, or B[a]P (5 μM) plus H.cordata (12.5, 25, or 50 mg/mL) for 24 h. The protein levels of pro-IL-1β (a and c) and IL-1β (a and d) in the cell lysates were detected after the same H.cordata treatments. (e and f) The protein levels of IL-1β in the culture supernatants were detected after treatment with vehicle, B[a]P (5 μM) alone, or B[a]P (5 μM) plus 2-undecanone (25, 50, or 100 μM) for 24 h. The protein levels of pro-IL-1β (e and g) and IL-1β (E and H) in the cell lysates were detected after the same 2-undecanone treatments. i The IL-1β levels in the plasma of mice were detected with an ELISA kit. The data represent the mean ± SD (n = 3). ^##p < 0.01 and ^###p < 0.001 compared with the control cells (given water) or control mice (given B[a]P + oil); ^**p < 0.01 and ^***p < 0.001 compared with the model cells (given B[a]P) or control mice (given B[a]P)