Fig. 8

H. cordata and 2-undecanone induce Nrf2-mediated expression of HO-1 and NQO-1 to reduce B[a]P-induced ROS overproduction. Nrf2 expression was silenced in BEAS-2B cells by transfection with one of two Nrf2-specific siRNAs (siNrf2-1 or siNrf2-2). The protein levels were measured by using Western blot analysis. a and b The protein levels of HO-1 were measured in cells exposed to H. cordata (25 mg/mL) or 2-undecanone (50 μM) in the presence or absence of siNrf2-1 or siNrf2-2 for 48 h. c and d) The protein levels of NQO-1 were measured in cells subjected to the same treatments. e and f Intracellular ROS levels were detected in cells subjected to the described treatments by using flow cytometry. ^*p < 0.05, ^**p< 0.01, and ^***p < 0.001 compared with the siCtrl group