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Fig. 9 | Journal of Experimental & Clinical Cancer Research

Fig. 9

From: Houttuynia cordata Thunb. and its bioactive compound 2-undecanone significantly suppress benzo(a)pyrene-induced lung tumorigenesis by activating the Nrf2-HO-1/NQO-1 signaling pathway

Fig. 9

The Nrf2-HO-1/NQO-1 signaling pathway mediates the protective effects of H. cordata and 2-undecanone against B[a]P-induced DNA damage, inflammation and cytotoxicity. The protein levels were measured by using Western blot analysis. Cell viability was determined by MTT assay. a and b The protein levels of p-H2A.X were measured in cells exposed to H. cordata (25 mg/mL) or 2-undecanone (50 μM) in the presence or absence of siNrf2-1 or siNrf2-2 for 48 h. c and d The protein levels of IL-1β in cell culture supernatants were measured after treatment of cells with H. cordata (25 mg/mL) or 2-undecanone (50 μM) in the presence or absence of siNrf2-1 or siNrf2-2 for 24 h. e and f Cell viability was determined after treatment of cells with H. cordata (25 mg/mL) or 2-undecanone (50 μM) in the presence or absence of siNrf2-1 or siNrf2-2. **p < 0.01 and ***p< 0.001 compared with the siCtrl group

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