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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Antitumor activity of dual blockade of PD-L1 and MEK in NSCLC patients derived three-dimensional spheroid cultures

Fig. 1

a Western blot analysis of MEK, phospho-MEK, MAPK, phospho-MAPK and PD-L1 on protein lysates from NSCLC cell lines HCC827, PC9, H1975, H460, H358, H322, H1299 and BEAS-2B. β-actin was included as a loading control. b Protein expression from densitometric analysis performed on three separate experiments. c Real time qPCR analysis of PD-L1 mRNA expression. Results were normalized to 18S mRNA and analyzed by ΔCt method. One way ANOVA test followed by Tukey’s test were used for statistical analysis. * p < 0.05; **p < 0.01; ***p < 0.001

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