Fig. 3
a Real time qPCR analysis of IL-1 β, TNFα, IFNγ AND il-6 expressed by H460 and h1299 cell lines not treated (CTR) or treated with selumetinib (MEK-i). Results were normalized to 18S mRNA and analyzed by ΔCt method. One way ANOVA test followed by Tukey’s test were used for statistical analysis. * p < 0.05; **p < 0.01; ***p < 0.001. b-c) IFNγ and IL-12 mRNA expression levels in PBMCs obtained from healthy donors (b) or NSCLC patients (c), stimulated for 5 days with anti CD3/CD28 beads. Real time qPCR analysis was conducted on cells cultured in absence (CTR) or presence of selumetinib (MEK-i). Results were normalized to 18S mRNA and analyzed by ΔCt method. One way ANOVA test followed by Tukey’s test were used for statistical analysis. * p < 0.05; **p < 0.01; ***p < 0.001