Fig. 4

a Graphic representation of CD3, CD4, CD8 and CD14 expressed by PI negative and CD45 positive cells (up graph) and of PD-L1 and EPCAM expressed on CD45 and PI negative cells (down graph) obtained from lung biopsies digested with collagenase IV. The analysis was conducted by flow cytometry. b Schematic representation of biopsy enzymatic digestion; representative images of organoids obtained from each centrifugation (S1, S2, S3) are reported. Cells obtained from S1, S2 and S3 were analyzed by flow cytometric analysis. From PI negative and CD45 positive cells were analyzed markers like CD3, CD4, CD8, CD13, PD-1, CD11c and PD-L1. From cells negative for PI and CD45 were considered EPCAM+ cells. c Immunofluorescence analysis of spheroid stained with: DAPI, CD45 and Cytokeratin. d Graphic representation of MHC-I expressed on the surface of cancer cells before and after stimulation with IFNγ