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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: PTENP1/miR-20a/PTEN axis contributes to breast cancer progression by regulating PTEN via PI3K/AKT pathway

Fig. 2

Overexpression of PTENP1 suppresses BC progression. a CCK8 assays were conducted to detect the growth of MDA-MB-231 and MCF-7/ADR cells. b The inhibitory proliferative effect of PTENP1 on MDA-MB-231 and MCF-7/ADR cells was determined by colony formation assay. c The proliferation of BC cells was measured by Edu staining. Green fluorescence: Edu, blue fluorescence: DAPI (Scale bar = 20 μm). d Ki67 staining also showed the repressed proliferation of PTENP1 transfected BC cells. Red fluorescence: Ki67, blue fluorescence: DAPI (Scale bar = 20 μm). e The migration and invasion of transfected MDA-MB-231 cells were suppressed by transwell experiment (Scale bar = 20 μm). f The chemoresistance to ADR of MCF-7/ADR cells was assessed by CCK8 assays. g The IC50 value was computed in the transfected MCF-7/ADR cells. h With ADR treatment, the colony formation of PTENP1 transfected MCF-7/ADR cells was more inhibited. i With ADR treatment, the apoptotic rate was determined by flow cytometry. j The mitochondrial membrane potential was measured by JC-1 staining. Green fluorescence: the monomer, red fluorescence: the J-aggregates, orange fluorescence: merged photo (Scale bar = 20 μm). k The occurrence of apoptosis was further confirmed by TUNEL assay (Scale bar = 200 μm). l The expression of caspase-related apoptotic molecules was determined by western blot. m The tumorigenesis was detected by xenograft model. The nude mice treated with PTENP1 revealed smaller tumor volume compared to the control group. The tumor was further inhibited in response to ADR treatment. n The levels of PTEN and Ki67 were determined by IHC staining. Data are the means ± SD of triplicate determinants (*P < 0.05), (Scale bar = 200 μm)

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