Fig. 7

PTENP1/miR-20a/PTEN axis activates PI3K/Akt pathway in PI3K/AKT- mediated BC cell progression. a The main molecules of PI3K/Akt pathway were determined by western blot after transfection with miR-20a mimic or PTENP1. b The main molecules of PI3K/Akt pathway were measured with treatment of miR-20a inhibitor or siPTENP1. c The PI3K/Akt pathway was repressed by LY294002 and Akt siRNA. d Using colony formation assay and transwell assay, the attenuated proliferation and aggressiveness were shown in the cells transfected with LY294002, Akt siRNA or combination of siAkt and PTENP1. Data are means ± SD of three independent assays (*P < 0.05) (Scale bar = 20 μm)