Fig. 6
In vivo evidence of dietary-PEITC mediated p53P223L/V274F mutant reactivation and growth inhibition of DU145 tumors. a Representative images of DU145 prostate xenograft tumors in control (Left Panel) and PEITC-treated (Right Panel) animal groups. b Tumors volumes in animals. (***p ≤ 0.0001 and **p ≤ 0.003) (n = 14). c Animal weights (g) were measured weekly. d Representative images of tumor tissue stained for Ki67 and p53. All scale bars represents a size of 200 μm. e Percentage of Ki67 (*p ≤ 0.01) and p53 (***p ≤ 0.00005) positive cells in tumor tissue sections (n = 10). Results are expressed as ± SD. f Western blot analysis of p53 expression levels in the tumors from control and PEITC-treated animal groups. Blot shown is representative of the 12 tumor tissue lysates analyzed from each group. g qRT-PCR (n = 4) of p53 regulated genes in PEITC and control animal groups. Results are expressed as ± SD. (***p ≤ 0.0003). h Western blot of p21 and Bax expression in DU145 xenograft tumors in vivo