Fig. 1

USP3 expression in gastric cancer (GC) was associated with a poor prognosis. a Western blot analysis of USP3 levels in human GC tissues and adjacent nontumor tissues. Expression levels of USP3 were normalized to the expression level of GAPDH. b The expression of USP3 mRNA in immortalised gastric mucosal cell line GES-1 and gastric cancer cell lines AGS, BGC-823, MGC-803, HGC-27, MKN28 and SGC7901 as detected via quantitative real-time RT-PCR. The experiment was performed intriplicate. *, p > 0.05; ****, p < 0.001. c USP3 expression in 87 samples of either normal or cancerous gastric tissues was detected by TMA analysis. d As analyzed by IHC, the USP3 expression level in GC tissues was significantly higher than that observed in the corresponding adjacent nontumor tissues. **** p < 0.001. e Kaplan-Meier survival analysis of overall survival in all patients (e) according to USP3 expression. The log-rank test was used to calculate p values. Scale bars, 200 μm in C